TDO2 ELISA Kit

Catalog No. ABIN5659599

Product Details TDO2 ELISA Kit

Target: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Tryptophan-2,3-dioxygenase (TDO). No significant cross-reactivity or interference between Tryptophan-2,3-dioxygenase (TDO) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptophan-2,3-dioxygenase (TDO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tryptophan-2,3-dioxygenase (TDO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tryptophan-2,3-dioxygenase (TDO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tryptophan-2,3-dioxygenase (TDO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tryptophan-2,3-dioxygenase (TDO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tryptophan-2,3-dioxygenase (TDO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TDO2

Target: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Alternative Name: Tryptophan-2,3-dioxygenase (TDO2 Products)

Synonyms: Tdo2 ELISA Kit, tdo2l ELISA Kit, fi30d08 ELISA Kit, zgc:63488 ELISA Kit, wu:fi30d08 ELISA Kit, TDO2 ELISA Kit, fb62b10 ELISA Kit, tdo2 ELISA Kit, wu:fb62b10 ELISA Kit, zgc:103693 ELISA Kit, DDBDRAFT_0190495 ELISA Kit, DDBDRAFT_0231363 ELISA Kit, DDB_0190495 ELISA Kit, DDB_0231363 ELISA Kit, TDO ELISA Kit, TO ELISA Kit, TPH2 ELISA Kit, TRPO ELISA Kit, AA407491 ELISA Kit, chky ELISA Kit, Tdo ELISA Kit, tryptophan 2,3-dioxygenase b ELISA Kit, tryptophan 2,3-dioxygenase ELISA Kit, tryptophan 2,3-dioxygenase a ELISA Kit, tryptophan 2,3-dioxygenase L homeolog ELISA Kit, tdo2b ELISA Kit, tdo2 ELISA Kit, TDO2 ELISA Kit, MADE_02822 ELISA Kit, CtCNB1_3657 ELISA Kit, tdo2a ELISA Kit, CNC04830 ELISA Kit, tdo ELISA Kit, Mrub_2119 ELISA Kit, Deipr_2235 ELISA Kit, Acav_1166 ELISA Kit, Mesop_4278 ELISA Kit, Tdo2 ELISA Kit, tdo2.L ELISA Kit

Background:

Gene Name: Tryptophan-2,3-dioxygenase

Gene Aliases: TDO2, TPH2, TO, TRPO, Tryptophanase, Tryptophan oxygenase

Gene ID: 6999

UniProt: P48775