TPM3 ELISA Kit

Catalog No. ABIN5659551

Product Details TPM3 ELISA Kit

Target: TPM3 (Tropomyosin 3 (TPM3))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Tropomyosin 3 (TPM3). No significant cross-reactivity or interference between Tropomyosin 3 (TPM3) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tropomyosin 3 (TPM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tropomyosin 3 (TPM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tropomyosin 3 (TPM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tropomyosin 3 (TPM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tropomyosin 3 (TPM3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tropomyosin 3 (TPM3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TPM3

Target: TPM3 (Tropomyosin 3 (TPM3))

Alternative Name: Tropomyosin 3 (TPM3 Products)

Synonyms: CAPM1 ELISA Kit, CFTD ELISA Kit, NEM1 ELISA Kit, OK/SW-cl.5 ELISA Kit, TM-5 ELISA Kit, TM3 ELISA Kit, TM30 ELISA Kit, TM30nm ELISA Kit, TM5 ELISA Kit, TPMsk3 ELISA Kit, TRK ELISA Kit, hscp30 ELISA Kit, cb674 ELISA Kit, fa17b11 ELISA Kit, fb83b01 ELISA Kit, fk41e01 ELISA Kit, hm:zeh0298 ELISA Kit, wu:fa17b11 ELISA Kit, wu:fb83b01 ELISA Kit, wu:fk41e01 ELISA Kit, zeh0298 ELISA Kit, zgc:77592 ELISA Kit, TPM3 ELISA Kit, Tm5NM ELISA Kit, Tpm-5 ELISA Kit, Tpm5 ELISA Kit, Trop-5 ELISA Kit, gamma-TM ELISA Kit, hTM30nm ELISA Kit, hTMnm ELISA Kit, tm3 ELISA Kit, tpm3 ELISA Kit, tpm3.L ELISA Kit, tropomyosin 3 ELISA Kit, tropomyosin 3, gamma ELISA Kit, tropomyosin 3 S homeolog ELISA Kit, TPM3 ELISA Kit, tpm3 ELISA Kit, Tpm3 ELISA Kit, tpm3.S ELISA Kit

Background:

Gene Name: Tropomyosin 3

Gene Aliases: NEM1, TRK, OK/SW-cl.5, TM-5, TM3, TM30, TM30nm, TPMsk3, Hscp30, Gamma-tropomyosin

Gene ID: 59069

UniProt: P21107