TPI1 ELISA Kit

Catalog No. ABIN5659544

Product Details TPI1 ELISA Kit

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Triosephosphate Isomerase 1 (TPI1). No significant cross-reactivity or interference between Triosephosphate Isomerase 1 (TPI1) and analogues was observed.

Sensitivity: 0.252 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Triosephosphate Isomerase 1 (TPI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Triosephosphate Isomerase 1 (TPI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Triosephosphate Isomerase 1 (TPI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Triosephosphate Isomerase 1 (TPI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Triosephosphate Isomerase 1 (TPI1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Triosephosphate Isomerase 1 (TPI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TPI1

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Alternative Name: Triosephosphate Isomerase 1 (TPI1 Products)

Synonyms: TIM ELISA Kit, TPI ELISA Kit, TIM1 ELISA Kit, hTIM ELISA Kit, tpi ELISA Kit, tpi1 ELISA Kit, TPI1 ELISA Kit, AI255506 ELISA Kit, Tpi ELISA Kit, Tpi-1 ELISA Kit, GB17473 ELISA Kit, Tim ELISA Kit, CG2171 ELISA Kit, Dmel\\CG2171 ELISA Kit, ND14 ELISA Kit, TPIS ELISA Kit, sgk ELISA Kit, wstd ELISA Kit, DrTPI-A ELISA Kit, DrTPI-B ELISA Kit, TIM-B ELISA Kit, cb179 ELISA Kit, wu:fb51c02 ELISA Kit, triosephosphate isomerase 1 ELISA Kit, timeless circadian regulator ELISA Kit, triosephosphate isomerase 1 S homeolog ELISA Kit, triosephosphate isomerase ELISA Kit, triose-phosphate isomerase ELISA Kit, Triose-phosphate isomerase ELISA Kit, triose-phosphate isomerase, putative ELISA Kit, timeless circadian clock ELISA Kit, Triose phosphate isomerase ELISA Kit, Triosephosphate isomerase ELISA Kit, triosephosphate isomerase 1a ELISA Kit, triosephosphate isomerase 1b ELISA Kit, TPI1 ELISA Kit, TIMELESS ELISA Kit, tpi1 ELISA Kit, Tpi1 ELISA Kit, tpi1.S ELISA Kit, Tpi ELISA Kit, tpiA ELISA Kit, CNE05000 ELISA Kit, Fnod_0738 ELISA Kit, Tlet_0996 ELISA Kit, SARE_RS16215 ELISA Kit, PKH_126640 ELISA Kit, Timeless ELISA Kit, tpi-1 ELISA Kit, tpi1a ELISA Kit, tpi1b ELISA Kit

Background:

Gene Name: Triosephosphate Isomerase 1

Gene Aliases: TIM, Triose-phosphate isomerase

Gene ID: 7167

UniProt: P60174

Pathways: Cell RedoxHomeostasis