TREH ELISA Kit

Catalog No. ABIN5659526

Product Details TREH ELISA Kit

Target: TREH (Trehalase (Brush-Border Membrane Glycoprotein) (TREH))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Trehalase (TREH). No significant cross-reactivity or interference between Trehalase (TREH) and analogues was observed.

Sensitivity: 0.216 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trehalase (TREH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Trehalase (TREH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Trehalase (TREH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Trehalase (TREH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Trehalase (TREH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Trehalase (TREH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TREH

Target: TREH (Trehalase (Brush-Border Membrane Glycoprotein) (TREH))

Alternative Name: Trehalase (TREH Products)

Synonyms: 87D3T ELISA Kit, CG9364 ELISA Kit, Dmel\\CG9364 ELISA Kit, ESTS:87D3T ELISA Kit, TRE ELISA Kit, TREH ELISA Kit, Tre ELISA Kit, anon-EST:Posey159 ELISA Kit, anon-sts38 ELISA Kit, GB16316 ELISA Kit, TREA ELISA Kit, 2210412M19Rik ELISA Kit, Trehalase ELISA Kit, trehalase ELISA Kit, trehalase (brush-border membrane glycoprotein) ELISA Kit, Treh ELISA Kit, LOC693027 ELISA Kit, LOC410795 ELISA Kit, TREH ELISA Kit

Background:

Gene Name: Trehalase

Gene Aliases: TRE, TREA, Brush Border Membrane Glycoprotein, Alpha,Alpha-Trehalase, Alpha,Alpha-Trehalose Glucohydrolase

Gene ID: 58866

UniProt: Q9JLT2