TOM70 ELISA Kit

Catalog No. ABIN5659495

Product Details TOM70 ELISA Kit

Target: TOM70 (TOMM70A) (Translocase of Outer Mitochondrial Membrane 70 (TOMM70A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A). No significant cross-reactivity or interference between Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) and analogues was observed.

Sensitivity: 0.101 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TOM70

Target: TOM70 (TOMM70A) (Translocase of Outer Mitochondrial Membrane 70 (TOMM70A))

Alternative Name: Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A Products)

Synonyms: MGC68780 ELISA Kit, TOMM70A ELISA Kit, Tom70 ELISA Kit, KIAA0719 ELISA Kit, MGC73188 ELISA Kit, zgc:73188 ELISA Kit, wu:fj58b04 ELISA Kit, CG6756 ELISA Kit, Dmel\\CG6756 ELISA Kit, dtom70 ELISA Kit, GB13743 ELISA Kit, TOM70 ELISA Kit, 2610044B22Rik ELISA Kit, D16Ium22 ELISA Kit, D16Ium22e ELISA Kit, D16Wsu109e ELISA Kit, mKIAA0719 ELISA Kit, to translocase of outer mitochondrial membrane 70 S homeolog ELISA Kit, translocase of outer mitochondrial membrane 70 ELISA Kit, translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae) ELISA Kit, Translocase of outer membrane 70 ELISA Kit, mitochondrial import receptor subunit TOM70 ELISA Kit, translocase of outer mitochondrial membrane 70 homolog A (yeast) ELISA Kit, tomm70.S ELISA Kit, TOMM70 ELISA Kit, tomm70a ELISA Kit, tomm70 ELISA Kit, Tom70 ELISA Kit, LOC726081 ELISA Kit, Tomm70 ELISA Kit, Tomm70a ELISA Kit

Background:

Gene Name: Translocase Of Outer Mitochondrial Membrane 70A

Gene Aliases: TOM70A, Mitochondrial precursor proteins import receptor, Mitochondrial import receptor subunit TOM70, Translocase of outer membrane 70 kDa subunit

Gene ID: 9868

UniProt: O94826

Pathways: SARS-CoV-2 Protein Interactome