Transgelin ELISA Kit

Catalog No. ABIN5659469

Product Details Transgelin ELISA Kit

Target: Transgelin (TAGLN)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Transgelin (TAGLN). No significant cross-reactivity or interference between Transgelin (TAGLN) and analogues was observed.

Sensitivity: 0.065 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transgelin (TAGLN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transgelin (TAGLN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transgelin (TAGLN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transgelin (TAGLN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transgelin (TAGLN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transgelin (TAGLN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Transgelin

Target: Transgelin (TAGLN)

Alternative Name: Transgelin (TAGLN Products)

Synonyms: MGC68550 ELISA Kit, sm22 ELISA Kit, smcc ELISA Kit, tagln1 ELISA Kit, ws3-10 ELISA Kit, MGC107855 ELISA Kit, SM22 ELISA Kit, SMCC ELISA Kit, TAGLN1 ELISA Kit, WS3-10 ELISA Kit, Sm22 ELISA Kit, Sm22a ELISA Kit, Ws310 ELISA Kit, SM22A ELISA Kit, zgc:153186 ELISA Kit, transgelin L homeolog ELISA Kit, transgelin ELISA Kit, tagln.L ELISA Kit, tagln ELISA Kit, LOC732906 ELISA Kit, TAGLN ELISA Kit, tagl ELISA Kit, LOC100009332 ELISA Kit, Tagln ELISA Kit

Background:

Gene Name: Transgelin

Gene Aliases: SMCC, SM22, TAGLN1, WS3-10, Transgelin Variant 2, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha

Gene ID: 6876

UniProt: Q01995