TRA2B ELISA Kit

Catalog No. ABIN5659414

Product Details TRA2B ELISA Kit

Target: TRA2B (Transformer 2 beta Homolog (TRA2B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Transformer 2 Beta (TRA2b). No significant cross-reactivity or interference between Transformer 2 Beta (TRA2b) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transformer 2 Beta (TRA2b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transformer 2 Beta (TRA2b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transformer 2 Beta (TRA2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transformer 2 Beta (TRA2b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transformer 2 Beta (TRA2b) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transformer 2 Beta (TRA2b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TRA2B

Target: TRA2B (Transformer 2 beta Homolog (TRA2B))

Alternative Name: Transformer 2 Beta (TRA2B Products)

Synonyms: sfrs10 ELISA Kit, SFRS10 ELISA Kit, Htra2-beta ELISA Kit, SRFS10 ELISA Kit, TRA2-BETA ELISA Kit, TRAN2B ELISA Kit, 5730405G21Rik ELISA Kit, D16Ertd266e ELISA Kit, SIG-41 ELISA Kit, Sfrs10 ELISA Kit, Silg41 ELISA Kit, TRA2beta ELISA Kit, Srsf10 ELISA Kit, zgc:56612 ELISA Kit, transformer 2 beta homolog (Drosophila) ELISA Kit, transformer 2 beta homolog L homeolog ELISA Kit, transformer 2 beta homolog ELISA Kit, tra2b ELISA Kit, tra2b.L ELISA Kit, TRA2B ELISA Kit, Tra2b ELISA Kit

Background:

Gene Name: Transformer 2 Beta

Gene Aliases: SRFS10, Htra2-beta, TRA2-BETA, TRA2B, Splicing Factor,Arginine/Serine-Rich 10, Transformer-2 protein homolog B

Gene ID: 6434

UniProt: P62995