TCF7L2 ELISA Kit

Catalog No. ABIN5659392

Product Details TCF7L2 ELISA Kit

Target: TCF7L2 (Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Transcription Factor 7 Like Protein 2 (TCF7L2). No significant cross-reactivity or interference between Transcription Factor 7 Like Protein 2 (TCF7L2) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Factor 7 Like Protein 2 (TCF7L2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Factor 7 Like Protein 2 (TCF7L2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Factor 7 Like Protein 2 (TCF7L2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transcription Factor 7 Like Protein 2 (TCF7L2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transcription Factor 7 Like Protein 2 (TCF7L2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transcription Factor 7 Like Protein 2 (TCF7L2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TCF7L2

Target: TCF7L2 (Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2))

Alternative Name: Transcription Factor 7 Like Protein 2 (TCF7L2 Products)

Synonyms: TCF4B ELISA Kit, TCF4E ELISA Kit, Tcf-4 ELISA Kit, Tcf4 ELISA Kit, mTcf-4B ELISA Kit, mTcf-4E ELISA Kit, tcf4 ELISA Kit, XTCF-4 ELISA Kit, tcf4e ELISA Kit, TCF4 ELISA Kit, TCF-4 ELISA Kit, TCFL2 ELISA Kit, transcription factor 7 like 2 ELISA Kit, transcription factor 7-like 2 ELISA Kit, transcription factor 7 like 2, T cell specific, HMG box ELISA Kit, transcription factor 7 like 2 L homeolog ELISA Kit, transcription factor 7-like 2 (T-cell specific, HMG-box) ELISA Kit, TCF7L2 ELISA Kit, LOC100545943 ELISA Kit, Tcf7l2 ELISA Kit, tcf7l2 ELISA Kit, tcf7l2.L ELISA Kit

Background:

Gene Name: Transcription Factor 7 Like Protein 2

Gene Aliases: TCF4, HMG box transcription factor 4

Pathways: WNT Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus