TALDO1 ELISA Kit

Catalog No. ABIN5659388

Product Details TALDO1 ELISA Kit

Target: TALDO1 (Transaldolase 1 (TALDO1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Transaldolase (TALDO1). No significant cross-reactivity or interference between Transaldolase (TALDO1) and analogues was observed.

Sensitivity: 0.067 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transaldolase (TALDO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transaldolase (TALDO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transaldolase (TALDO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transaldolase (TALDO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transaldolase (TALDO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transaldolase (TALDO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TALDO1

Target: TALDO1 (Transaldolase 1 (TALDO1))

Alternative Name: Transaldolase (TALDO1 Products)

Synonyms: TAL ELISA Kit, TAL-H ELISA Kit, TALDOR ELISA Kit, TALH ELISA Kit, Tal ELISA Kit, wu:fc32g02 ELISA Kit, zgc:56232 ELISA Kit, zgc:66054 ELISA Kit, EPS8L2 ELISA Kit, transaldolase ELISA Kit, TALDO1 ELISA Kit, taldo1 ELISA Kit, An07g03850 ELISA Kit, AO090020000520 ELISA Kit, transaldolase 1 ELISA Kit, transaldolase ELISA Kit, transaldolase 1 L homeolog ELISA Kit, Transaldolase 1 ELISA Kit, TALDO1 ELISA Kit, Taldo1 ELISA Kit, taldo1 ELISA Kit, LOC475937 ELISA Kit, taldo1.L ELISA Kit, talB ELISA Kit, RB3193 ELISA Kit, ANI_1_472064 ELISA Kit, AOR_1_906084 ELISA Kit, TAL1_1 ELISA Kit, Mrub_1361 ELISA Kit, Arnit_2857 ELISA Kit, Mesil_2037 ELISA Kit, Slip_2309 ELISA Kit, Acear_0056 ELISA Kit, Thena_1180 ELISA Kit, Halhy_0294 ELISA Kit, Mahau_0255 ELISA Kit, Isova_1495 ELISA Kit, talA.1 ELISA Kit, FsymDg_1707 ELISA Kit, Mesop_1203 ELISA Kit, Thein_0556 ELISA Kit

Background:

Gene Name: Transaldolase

Gene Aliases: TAL, TALDO, TALDOR, Transaldolase 1