TOP2 ELISA Kit

Catalog No. ABIN5659381

Product Details TOP2 ELISA Kit

Target: TOP2 (DNA Topoisomerase II (TOP2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Topoisomerase II (TOP2). No significant cross-reactivity or interference between Topoisomerase II (TOP2) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Topoisomerase II (TOP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Topoisomerase II (TOP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Topoisomerase II (TOP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Topoisomerase II (TOP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Topoisomerase II (TOP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Topoisomerase II (TOP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TOP2

Target: TOP2 (DNA Topoisomerase II (TOP2))

Alternative Name: Topoisomerase II (TOP2 Products)

Synonyms: Topoii ELISA Kit, ptr11 ELISA Kit, DDBDRAFT_0191045 ELISA Kit, DDBDRAFT_0231510 ELISA Kit, DDB_0191045 ELISA Kit, DDB_0231510 ELISA Kit, CG10223 ELISA Kit, CHRAC ELISA Kit, Dmel\\CG10223 ELISA Kit, TOP2 ELISA Kit, TOPO II ELISA Kit, TOPOII ELISA Kit, TopII ELISA Kit, Topo II ELISA Kit, TopoII ELISA Kit, dCHRAC ELISA Kit, dTopoII ELISA Kit, top2 ELISA Kit, topo II ELISA Kit, topoII ELISA Kit, topoisomerase II ELISA Kit, DNA topoisomerase II ELISA Kit, DNA topoisomerase 2 ELISA Kit, DNA topoisomerase ii ELISA Kit, type IIA topoisomerase; DNA gyrase topoisomerase IV B subunit ELISA Kit, Topoisomerase 2 ELISA Kit, TOP2 ELISA Kit, A583L ELISA Kit, top2 ELISA Kit, CNA03600 ELISA Kit, Tb09.160.4090 ELISA Kit, Tb11.01.3390 ELISA Kit, LMJF_28_2280 ELISA Kit, Top2 ELISA Kit

Background:

Gene Name: Topoisomerase II

Gene Aliases: DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme

Gene ID: 360243

UniProt: P41516

Pathways: Mitotic G1-G1/S Phases