Titin ELISA Kit

Catalog No. ABIN5659335

Product Details Titin ELISA Kit

Target: Titin (TTN)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Titin (TTN). No significant cross-reactivity or interference between Titin (TTN) and analogues was observed.

Sensitivity: 0.116 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Titin (TTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Titin (TTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Titin (TTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Titin (TTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Titin (TTN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Titin (TTN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Titin

Target: Titin (TTN)

Alternative Name: Titin (TTN Products)

Synonyms: CMD1G ELISA Kit, CMH9 ELISA Kit, CMPD4 ELISA Kit, EOMFC ELISA Kit, HMERF ELISA Kit, LGMD2J ELISA Kit, MYLK5 ELISA Kit, TMD ELISA Kit, 1100001C23Rik ELISA Kit, 2310036G12Rik ELISA Kit, 2310057K23Rik ELISA Kit, 2310074I15Rik ELISA Kit, AF006999 ELISA Kit, AV006427 ELISA Kit, D330041I19Rik ELISA Kit, D830007G01Rik ELISA Kit, L56 ELISA Kit, mdm ELISA Kit, shru ELISA Kit, UNC-22 ELISA Kit, titin ELISA Kit, TTN ELISA Kit, Ttn ELISA Kit, titin ELISA Kit, TTNLOC100620261 ELISA Kit

Background:

Gene Name: Titin

Gene Aliases: CMD1G, CMH9, CMPD4, HMERF, LGMD2J, TMD, Cardiomyopathy,Dilated 1G(Autosomal Dominant)

Gene ID: 7273

UniProt: Q8WZ42