Tissue Polypeptide Specific Antigen CLIA Kit
TPS
Reactivity: Human
Chemiluminescent
Sandwich ELISA
312.5 pg/mL - 20000 pg/mL
Plasma, Serum
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- Target See all Tissue Polypeptide Specific Antigen (TPS) products
- Tissue Polypeptide Specific Antigen (TPS)
- Reactivity
- Human
- Detection Method
- Chemiluminescent
- Method Type
- Sandwich ELISA
- Detection Range
- 312.5 pg/mL - 20000 pg/mL
- Minimum Detection Limit
- 312.5 pg/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Tissue Polypeptide Specific Antigen (TPS). No significant cross-reactivity or interference between Tissue Polypeptide Specific Antigen (TPS) and analogues was observed.
- Sensitivity
- 12.57 pg/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- The microplate provided in this kit has been pre-coated with an antibody specific to Tissue Polypeptide Specific Antigen (TPS). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tissue Polypeptide Specific Antigen (TPS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tissue Polypeptide Specific Antigen (TPS) level in the sample or standard.,
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Polypeptide Specific Antigen (TPS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Polypeptide Specific Antigen (TPS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all Tissue Polypeptide Specific Antigen (TPS) products
- Tissue Polypeptide Specific Antigen (TPS)
- Alternative Name
- Tissue Polypeptide Specific Antigen (TPS Products)
- Background
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Gene Name: Tissue Polypeptide Specific Antigen
Gene Aliases: Specific M3 Epitope Of TPA
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