THRA ELISA Kit

Catalog No. ABIN5659227

Product Details THRA ELISA Kit

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Thyroid Hormone Receptor Alpha (THRa). No significant cross-reactivity or interference between Thyroid Hormone Receptor Alpha (THRa) and analogues was observed.

Sensitivity: 22.5 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Hormone Receptor Alpha (THRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroid Hormone Receptor Alpha (THRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Hormone Receptor Alpha (THRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Hormone Receptor Alpha (THRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Hormone Receptor Alpha (THRa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Hormone Receptor Alpha (THRa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for THRA

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Alternative Name: Thyroid Hormone Receptor Alpha (THRA Products)

Synonyms: AR7 ELISA Kit, CHNG6 ELISA Kit, EAR7 ELISA Kit, ERB-T-1 ELISA Kit, ERBA ELISA Kit, ERBA1 ELISA Kit, NR1A1 ELISA Kit, THRA1 ELISA Kit, THRA2 ELISA Kit, c-ERBA-1 ELISA Kit, C-ERBA-ALPHA ELISA Kit, TRalpha ELISA Kit, c-erba ELISA Kit, erba ELISA Kit, nr1a1-A ELISA Kit, thra-A ELISA Kit, thra1 ELISA Kit, TR[a]1 ELISA Kit, nr1a1 ELISA Kit, thra ELISA Kit, thral ELISA Kit, tra ELISA Kit, tra1 ELISA Kit, wu:fb59g07 ELISA Kit, Thra1 ELISA Kit, 6430529J03Rik ELISA Kit, AW259572 ELISA Kit, Erba ELISA Kit, Nr1a1 ELISA Kit, Rvr ELISA Kit, T3R[a] ELISA Kit, T3Ralpha ELISA Kit, Thra2 ELISA Kit, C-ERBA ALPHA1 ELISA Kit, Thra ELISA Kit, fTHRalphaA ELISA Kit, nr1a1-a ELISA Kit, thyroid hormone receptor, alpha ELISA Kit, thyroid hormone receptor, alpha L homeolog ELISA Kit, thyroid hormone receptor alpha a ELISA Kit, thyroid hormone receptor alpha ELISA Kit, thyroid hormone receptor alpha-A ELISA Kit, THRA ELISA Kit, thra.L ELISA Kit, thraa ELISA Kit, Thra ELISA Kit, tr-alpha ELISA Kit, LOC109641414 ELISA Kit

Background:

Gene Name: Thyroid Hormone Receptor Alpha

Gene Aliases: THR-A, NR1A1, AR7, EAR7, ERB-T1, ERBA1, NR1-A1, THRA1, THRA2, c-ERBA1, Erythroblastic Leukemia Viral Oncogene Homolog, Nuclear Receptor Subfamily 1,Group A,Member 1

Gene ID: 7067

UniProt: P10827

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Skeletal Muscle Fiber Development