TMSB4Y ELISA Kit

Catalog No. ABIN5659217

Product Details TMSB4Y ELISA Kit

Target: TMSB4Y (Thymosin beta 4, Y-Linked (TMSB4Y))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 123.46 ng/mL - 10000 ng/mL

Minimum Detection Limit: 123.46 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Thymosin Beta 4, Y-Chromosomal (Tb4Y). No significant cross-reactivity or interference between Thymosin Beta 4, Y-Chromosomal (Tb4Y) and analogues was observed.

Sensitivity: 42.2 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thymosin Beta 4, Y-Chromosomal (Tb4Y) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thymosin Beta 4, Y-Chromosomal (Tb4Y) and unlabeled Thymosin Beta 4, Y-Chromosomal (Tb4Y) (Standards or samples) with the pre-coated antibody specific to Thymosin Beta 4, Y-Chromosomal (Tb4Y). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thymosin Beta 4, Y-Chromosomal (Tb4Y) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thymosin Beta 4, Y-Chromosomal (Tb4Y) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymosin Beta 4, Y-Chromosomal (Tb4Y) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymosin Beta 4, Y-Chromosomal (Tb4Y) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TMSB4Y

Target: TMSB4Y (Thymosin beta 4, Y-Linked (TMSB4Y))

Alternative Name: Thymosin Beta 4, Y-Chromosomal (TMSB4Y Products)

Synonyms: TB4Y ELISA Kit, thymosin beta 4, Y-linked ELISA Kit, TMSB4Y ELISA Kit

Background:

Gene Name: Thymosin Beta 4, Y-Chromosomal

Gene Aliases: TMSB4Y, Thymosin,Beta 4,Y-Linked

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location