Thymidine Phosphorylase ELISA Kit

Catalog No. ABIN5659211

Product Details Thymidine Phosphorylase ELISA Kit

Target: Thymidine Phosphorylase (TYMP)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Thymidine Phosphorylase (TP). No significant cross-reactivity or interference between Thymidine Phosphorylase (TP) and analogues was observed.

Sensitivity: 0.115 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymidine Phosphorylase (TP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymidine Phosphorylase (TP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymidine Phosphorylase (TP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymidine Phosphorylase (TP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymidine Phosphorylase (TP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymidine Phosphorylase (TP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Thymidine Phosphorylase

Target: Thymidine Phosphorylase (TYMP)

Alternative Name: Thymidine Phosphorylase (TYMP Products)

Synonyms: ECGF ELISA Kit, ECGF1 ELISA Kit, MEDPS1 ELISA Kit, MNGIE ELISA Kit, MTDPS1 ELISA Kit, PDECGF ELISA Kit, TP ELISA Kit, hPD-ECGF ELISA Kit, 2900072D10Rik ELISA Kit, Ecgf1 ELISA Kit, PD-ECGF ELISA Kit, Pdgfec ELISA Kit, typh ELISA Kit, thymidine phosphorylase ELISA Kit, thymidine phosphorylase DeoA ELISA Kit, AMP phosphorylase ELISA Kit, Thymidine phosphorylase ELISA Kit, TYMP ELISA Kit, Tymp ELISA Kit, deoA ELISA Kit, Dd586_0614 ELISA Kit, Kvar_4413 ELISA Kit, NMAG_RS19330 ELISA Kit, Snov_1187 ELISA Kit, Fbal_2856 ELISA Kit, Ftrac_2071 ELISA Kit, Intca_1216 ELISA Kit, ARCVE_RS10415 ELISA Kit, Sphch_4174 ELISA Kit, Sinme_3461 ELISA Kit, METOK_RS06355 ELISA Kit, Mesop_1831 ELISA Kit, typh ELISA Kit

Background:

Gene Name: Thymidine Phosphorylase

Gene Aliases: TYMP, PDECGF, ECGF1, MNGIE, TP, HPD-ECGF, TdRPase, Platelet-Derived Endothelial Cell Growth Factor, Gliostatin

Gene ID: 72962

UniProt: Q99N42

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2