Thimet Oligopeptidase 1 ELISA Kit

Catalog No. ABIN5659144

Product Details Thimet Oligopeptidase 1 ELISA Kit

Target: Thimet Oligopeptidase 1 (THOP1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Thimet Oligopeptidase 1 (THOP1). No significant cross-reactivity or interference between Thimet Oligopeptidase 1 (THOP1) and analogues was observed.

Sensitivity: 0.63 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thimet Oligopeptidase 1 (THOP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thimet Oligopeptidase 1 (THOP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thimet Oligopeptidase 1 (THOP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thimet Oligopeptidase 1 (THOP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thimet Oligopeptidase 1 (THOP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thimet Oligopeptidase 1 (THOP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Thimet Oligopeptidase 1

Target: Thimet Oligopeptidase 1 (THOP1)

Alternative Name: Thimet Oligopeptidase 1 (THOP1 Products)

Synonyms: EP24.15 ELISA Kit, MEPD_HUMAN ELISA Kit, MP78 ELISA Kit, TOP ELISA Kit, AI131655 ELISA Kit, AI327041 ELISA Kit, THOP1 ELISA Kit, thop1 ELISA Kit, MGC147297 ELISA Kit, LOC397806 ELISA Kit, mepd ELISA Kit, thimet oligopeptidase 1 ELISA Kit, thimet oligopeptidase 1 L homeolog ELISA Kit, thimet oligopeptidase ELISA Kit, peptidase ELISA Kit, Thimet oligopeptidase ELISA Kit, THOP1 ELISA Kit, Thop1 ELISA Kit, thop1 ELISA Kit, thop1.L ELISA Kit, Tc00.1047053506411.10 ELISA Kit, Tc00.1047053511237.10 ELISA Kit, Noca_4613 ELISA Kit, ANI_1_1400064 ELISA Kit, MBOO_RS10705 ELISA Kit, AOR_1_880174 ELISA Kit, AnaeK_0425 ELISA Kit, MPAL_RS03920 ELISA Kit, Hoch_5061 ELISA Kit, mepd ELISA Kit

Background:

Gene Name: Thimet Oligopeptidase 1

Gene Aliases: EP24.15, MEPD_HUMAN, MP78, Endopeptidase 24.15

Gene ID: 7064

UniProt: P52888