TNKS ELISA Kit

Catalog No. ABIN5659090

Product Details TNKS ELISA Kit

Target: TNKS (Tankyrase, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase (TNKS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Tankyrase 1 (TNKS1). No significant cross-reactivity or interference between Tankyrase 1 (TNKS1) and analogues was observed.

Sensitivity: 0.115 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tankyrase 1 (TNKS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tankyrase 1 (TNKS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tankyrase 1 (TNKS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tankyrase 1 (TNKS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tankyrase 1 (TNKS1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tankyrase 1 (TNKS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TNKS

Target: TNKS (Tankyrase, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase (TNKS))

Alternative Name: Tankyrase 1 (TNKS Products)

Synonyms: wu:fe02c12 ELISA Kit, ARTD5 ELISA Kit, PARP-5a ELISA Kit, PARP5A ELISA Kit, PARPL ELISA Kit, TIN1 ELISA Kit, TINF1 ELISA Kit, TNKS1 ELISA Kit, pART5 ELISA Kit, 4930554K12Rik ELISA Kit, AI662855 ELISA Kit, C86528 ELISA Kit, D130072O21Rik ELISA Kit, TANK1 ELISA Kit, mTNKS1 ELISA Kit, tankyrase-1 ELISA Kit, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b ELISA Kit, tankyrase ELISA Kit, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase ELISA Kit, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 S homeolog ELISA Kit, tnksb ELISA Kit, TNKS ELISA Kit, Tnks ELISA Kit, tnks2.S ELISA Kit

Background:

Gene Name: Tankyrase 1

Gene Aliases: TNKS, ARTD5, PARPL, PARP-5a, PARP5A, TIN1, TINF1, TNKS-1, ADP-ribosyltransferase diphtheria toxin-like 5, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase