TAC1 ELISA Kit

Catalog No. ABIN5659083

Product Details TAC1 ELISA Kit

Target: TAC1 (Tachykinin, Precursor 1 (TAC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 123.46 pg/mL - 10000 pg/mL

Minimum Detection Limit: 123.46 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Tachykinin, Precursor 1 (TAC1). No significant cross-reactivity or interference between Tachykinin, Precursor 1 (TAC1) and analogues was observed.

Sensitivity: 52.8 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tachykinin, Precursor 1 (TAC1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tachykinin, Precursor 1 (TAC1) and unlabeled Tachykinin, Precursor 1 (TAC1) (Standards or samples) with the pre-coated antibody specific to Tachykinin, Precursor 1 (TAC1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tachykinin, Precursor 1 (TAC1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tachykinin, Precursor 1 (TAC1) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tachykinin, Precursor 1 (TAC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tachykinin, Precursor 1 (TAC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TAC1

Target: TAC1 (Tachykinin, Precursor 1 (TAC1))

Alternative Name: Tachykinin, Precursor 1 (TAC1 Products)

Synonyms: Hs.2563 ELISA Kit, NK2 ELISA Kit, NKNA ELISA Kit, NPK ELISA Kit, TAC2 ELISA Kit, PPTA3 ELISA Kit, Ppt5fl ELISA Kit, RATPPTA3 ELISA Kit, TAC ELISA Kit, TAC1 ELISA Kit, 4930528L02Rik ELISA Kit, NK-1 ELISA Kit, NK1 ELISA Kit, Nkna ELISA Kit, PPT-A ELISA Kit, PPTA ELISA Kit, SP ELISA Kit, PPT ELISA Kit, beta-PPT-A ELISA Kit, NKA ELISA Kit, nk2 ELISA Kit, nkna ELISA Kit, npk ELISA Kit, tac1 ELISA Kit, tac2 ELISA Kit, tachykinin ELISA Kit, tachykinin precursor 1 ELISA Kit, tachykinin, precursor 1 ELISA Kit, tachykinin 1 ELISA Kit, tachykinin precursor 1 L homeolog ELISA Kit, TAC1 ELISA Kit, Tac1 ELISA Kit, tac1 ELISA Kit, tac1.L ELISA Kit

Background:

Gene Name: Tachykinin, Precursor 1

Gene Aliases: NK2, NKNA, NKA, TAC2, •,PPT, Protachykinin-1, Substance P, Neurokinin A, Neuromedin L, Substance K, Neuropeptide K, Neuropeptide gamma, C-terminal-flanking peptide