STX1A ELISA Kit

Catalog No. ABIN5659067

Product Details STX1A ELISA Kit

Target: STX1A (Syntaxin 1A (Brain) (STX1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Syntaxin 1A, Brain (STX1A). No significant cross-reactivity or interference between Syntaxin 1A, Brain (STX1A) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Syntaxin 1A, Brain (STX1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Syntaxin 1A, Brain (STX1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Syntaxin 1A, Brain (STX1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Syntaxin 1A, Brain (STX1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Syntaxin 1A, Brain (STX1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Syntaxin 1A, Brain (STX1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for STX1A

Target: STX1A (Syntaxin 1A (Brain) (STX1A))

Alternative Name: Syntaxin 1A, Brain (STX1A Products)

Synonyms: HPC-1 ELISA Kit, P35-1 ELISA Kit, STX1 ELISA Kit, SYN1A ELISA Kit, STX1A ELISA Kit, hpc-1 ELISA Kit, p35-1 ELISA Kit, stx1 ELISA Kit, sx1a ELISA Kit, syntaxin1a ELISA Kit, CG10716 ELISA Kit, CG18615 ELISA Kit, CG31136 ELISA Kit, CG5448 ELISA Kit, CT30033 ELISA Kit, Dm Syx1 ELISA Kit, DmSyx1A ELISA Kit, Dmel\\CG31136 ELISA Kit, SYX1A ELISA Kit, Syt1A ELISA Kit, Syx ELISA Kit, Syx-1A ELISA Kit, Syx1 ELISA Kit, Syx1a ELISA Kit, SyxA ELISA Kit, anon-EST:Gibbs4 ELISA Kit, anon-WO02059370.54 ELISA Kit, dSyx1 ELISA Kit, l(3)06737 ELISA Kit, synt ELISA Kit, syt-1A ELISA Kit, syx ELISA Kit, syx-1 ELISA Kit, syx-1A ELISA Kit, syx1 ELISA Kit, syx1A ELISA Kit, syntaxin 1A ELISA Kit, syntaxin 1A (brain) ELISA Kit, Syntaxin 1A ELISA Kit, syntaxin-1A ELISA Kit, STX1A ELISA Kit, Stx1a ELISA Kit, stx1a ELISA Kit, Syx1A ELISA Kit, EDI_005240 ELISA Kit, Tsp_07354 ELISA Kit

Background:

Gene Name: Syntaxin 1A, Brain

Gene Aliases: STX-1A, HPC-1, STX1, P35-1, Neuron-specific antigen HPC-1

Gene ID: 6804

UniProt: Q16623

Pathways: Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport