APLP2 ELISA Kit

Catalog No. ABIN5659015

Product Details APLP2 ELISA Kit

Target: APLP2 (Amyloid beta (A4) Precursor-Like Protein 2 (APLP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Suppression Of Tumorigenicity 14 (ST14). No significant cross-reactivity or interference between Suppression Of Tumorigenicity 14 (ST14) and analogues was observed.

Sensitivity: 0.61 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Suppression Of Tumorigenicity 14 (ST14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Suppression Of Tumorigenicity 14 (ST14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Suppression Of Tumorigenicity 14 (ST14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Suppression Of Tumorigenicity 14 (ST14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Suppression Of Tumorigenicity 14 (ST14) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Suppression Of Tumorigenicity 14 (ST14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for APLP2

Target: APLP2 (Amyloid beta (A4) Precursor-Like Protein 2 (APLP2))

Alternative Name: Suppression Of Tumorigenicity 14 (APLP2 Products)

Synonyms: HAI ELISA Kit, MT-SP1 ELISA Kit, MTSP1 ELISA Kit, PRSS14 ELISA Kit, SNC19 ELISA Kit, TADG15 ELISA Kit, TMPRSS14 ELISA Kit, APLP-2 ELISA Kit, APPH ELISA Kit, APPL2 ELISA Kit, CDEBP ELISA Kit, AI790698 ELISA Kit, Apph ELISA Kit, WALPLP2 ELISA Kit, aplp2 B ELISA Kit, APLP2 ELISA Kit, si:dkey-11k4.3 ELISA Kit, aplp2 A ELISA Kit, MGC86389 ELISA Kit, suppression of tumorigenicity 14 ELISA Kit, amyloid beta precursor like protein 2 ELISA Kit, amyloid beta (A4) precursor-like protein 2 ELISA Kit, amyloid beta (A4) precursor-like protein 2 S homeolog ELISA Kit, amyloid beta (A4) precursor-like protein 2 L homeolog ELISA Kit, ST14 ELISA Kit, APLP2 ELISA Kit, Aplp2 ELISA Kit, aplp2.S ELISA Kit, aplp2 ELISA Kit, aplp2.L ELISA Kit

Background:

Gene Name: Suppression Of Tumorigenicity 14

Gene Aliases: SNC19, HAI, MT-SP1, PRSS14, TADG15, Epithin, Matriptase, Prostamin, Membrane-type serine protease 1, Serine protease 14, Tumor-associated differentially-expressed gene 15

Pathways: EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Feeding Behaviour