SF3B3 ELISA Kit

Catalog No. ABIN5658926

Product Details SF3B3 ELISA Kit

Target: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Splicing Factor 3B Subunit 3 (SF3B3). No significant cross-reactivity or interference between Splicing Factor 3B Subunit 3 (SF3B3) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Splicing Factor 3B Subunit 3 (SF3B3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Splicing Factor 3B Subunit 3 (SF3B3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Splicing Factor 3B Subunit 3 (SF3B3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Splicing Factor 3B Subunit 3 (SF3B3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Splicing Factor 3B Subunit 3 (SF3B3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Splicing Factor 3B Subunit 3 (SF3B3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SF3B3

Target: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Alternative Name: Splicing Factor 3B Subunit 3 (SF3B3 Products)

Synonyms: GB11574 ELISA Kit, rse1 ELISA Kit, sap130 ELISA Kit, sf3b130 ELISA Kit, staf130 ELISA Kit, sf3b3 ELISA Kit, 1810061H24Rik ELISA Kit, 5730409A01Rik ELISA Kit, AA409318 ELISA Kit, D8Ertd633e ELISA Kit, RSE1 ELISA Kit, SAP130 ELISA Kit, mKIAA0017 ELISA Kit, ik:tdsubc_2b2 ELISA Kit, wu:fb81f05 ELISA Kit, xx:tdsubc_2b2 ELISA Kit, zgc:55440 ELISA Kit, SF3b130 ELISA Kit, STAF130 ELISA Kit, splicing factor 3b subunit 3 ELISA Kit, splicing factor 3B subunit 3 ELISA Kit, splicing factor 3b, subunit 3, 130kDa ELISA Kit, splicing factor 3b subunit 3 L homeolog ELISA Kit, splicing factor 3b, subunit 3 ELISA Kit, SF3B3 ELISA Kit, LOC550935 ELISA Kit, sf3b3 ELISA Kit, Sf3b3 ELISA Kit, LOC100178056 ELISA Kit, sf3b3.L ELISA Kit

Background:

Gene Name: Splicing Factor 3B Subunit 3

Gene Aliases: RSE1, SAP130, SF3b130, STAF130, Spliceosome-associated protein 130, Pre-mRNA-splicing factor SF3b 130 kDa subunit

Gene ID: 101943

UniProt: Q921M3