SGPL1 ELISA Kit

Catalog No. ABIN5658912

Product Details SGPL1 ELISA Kit

Target: SGPL1 (Sphingosine-1-Phosphate Lyase 1 (SGPL1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Sphingosine 1 Phosphate Lyase 1 (SGPL1). No significant cross-reactivity or interference between Sphingosine 1 Phosphate Lyase 1 (SGPL1) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Lyase 1 (SGPL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Lyase 1 (SGPL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Lyase 1 (SGPL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Lyase 1 (SGPL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sphingosine 1 Phosphate Lyase 1 (SGPL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sphingosine 1 Phosphate Lyase 1 (SGPL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SGPL1

Target: SGPL1 (Sphingosine-1-Phosphate Lyase 1 (SGPL1))

Alternative Name: Sphingosine 1 Phosphate Lyase 1 (SGPL1 Products)

Synonyms: SGPL1 ELISA Kit, zgc:162153 ELISA Kit, AI428538 ELISA Kit, D10Xrf456 ELISA Kit, S1PL ELISA Kit, Spl ELISA Kit, SPL ELISA Kit, Spgl1 ELISA Kit, spl ELISA Kit, sphingosine-1-phosphate lyase 1 ELISA Kit, sphingosine-1-phosphate lyase 1 L homeolog ELISA Kit, sphingosine phosphate lyase 1 ELISA Kit, SGPL1 ELISA Kit, sgpl1.L ELISA Kit, sgpl1 ELISA Kit, Sgpl1 ELISA Kit

Background:

Gene Name: Sphingosine 1 Phosphate Lyase 1

Gene Aliases: SPL, S1PL, Sphingosine-1-phosphate aldolase

Gene ID: 20397

UniProt: Q8R0X7

Pathways: Platelet-derived growth Factor Receptor Signaling