SORD ELISA Kit

Catalog No. ABIN5658893

Product Details SORD ELISA Kit

Target: SORD (Sorbitol Dehydrogenase (SORD))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Sorbitol Dehydrogenase (SDH). No significant cross-reactivity or interference between Sorbitol Dehydrogenase (SDH) and analogues was observed.

Sensitivity: 0.66 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorbitol Dehydrogenase (SDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorbitol Dehydrogenase (SDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorbitol Dehydrogenase (SDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sorbitol Dehydrogenase (SDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SORD

Target: SORD (Sorbitol Dehydrogenase (SORD))

Alternative Name: Sorbitol Dehydrogenase (SORD Products)

Synonyms: SORD1 ELISA Kit, Sdh-1 ELISA Kit, Sdh1 ELISA Kit, Sodh-1 ELISA Kit, SDH ELISA Kit, sord ELISA Kit, MGC83437 ELISA Kit, BmSDH ELISA Kit, PSPTO0363 ELISA Kit, SODH-1 ELISA Kit, RATSDHE1 ELISA Kit, SDH2 ELISA Kit, Sdh ELISA Kit, Sdhe1 ELISA Kit, TDH ELISA Kit, 4733401P19Rik ELISA Kit, AI836084 ELISA Kit, CGI-97 ELISA Kit, SDS ELISA Kit, zgc:63674 ELISA Kit, sorbitol dehydrogenase ELISA Kit, sorbitol dehydrogenase L homeolog ELISA Kit, Sorbitol dehydrogenase ELISA Kit, serine dehydratase ELISA Kit, SBDS ribosome maturation factor ELISA Kit, SORD ELISA Kit, Sord ELISA Kit, sord.L ELISA Kit, sord ELISA Kit, Sdh ELISA Kit, LOC733025 ELISA Kit, polS ELISA Kit, RS_RS10765 ELISA Kit, sdh ELISA Kit, smoS ELISA Kit, Rsph17029_1731 ELISA Kit, Srd ELISA Kit, RSKD131_1411 ELISA Kit, SDS ELISA Kit, Sds ELISA Kit, Sbds ELISA Kit

Background:

Gene Name: Sorbitol Dehydrogenase

Gene Aliases: SORD, L-iditol 2-dehydrogenase

Gene ID: 24788

UniProt: P27867