SLC3A2 ELISA Kit

Catalog No. ABIN5658861

Product Details SLC3A2 ELISA Kit

Target: SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 3, Member 2 (SLC3A2). No significant cross-reactivity or interference between Solute Carrier Family 3, Member 2 (SLC3A2) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 3, Member 2 (SLC3A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 3, Member 2 (SLC3A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 3, Member 2 (SLC3A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 3, Member 2 (SLC3A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 3, Member 2 (SLC3A2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 3, Member 2 (SLC3A2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SLC3A2

Target: SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))

Alternative Name: Solute Carrier Family 3, Member 2 (SLC3A2 Products)

Synonyms: 4F2 ELISA Kit, 4F2HC ELISA Kit, 4T2HC ELISA Kit, CD98 ELISA Kit, CD98HC ELISA Kit, MDU1 ELISA Kit, NACAE ELISA Kit, Mdu1 ELISA Kit, MGC53951 ELISA Kit, slc3a2 ELISA Kit, MGC53965 ELISA Kit, SLC3A2 ELISA Kit, AI314110 ELISA Kit, Cd98 ELISA Kit, Ly-10 ELISA Kit, Ly-m10 ELISA Kit, Ly10 ELISA Kit, Mgp-2hc ELISA Kit, fc65h12 ELISA Kit, wu:fc65h12 ELISA Kit, id:ibd1327 ELISA Kit, sb:cb519 ELISA Kit, wu:fe14a07 ELISA Kit, wu:fq18d12 ELISA Kit, zgc:55813 ELISA Kit, solute carrier family 3 member 2 ELISA Kit, solute carrier family 3 (amino acid transporter heavy chain), member 2 L homeolog ELISA Kit, solute carrier family 3 (amino acid transporter heavy chain), member 2 S homeolog ELISA Kit, solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 ELISA Kit, solute carrier family 3 (amino acid transporter heavy chain), member 2a ELISA Kit, solute carrier family 3 (amino acid transporter heavy chain), member 2b ELISA Kit, SLC3A2 ELISA Kit, Slc3a2 ELISA Kit, slc3a2.L ELISA Kit, slc3a2.S ELISA Kit, slc3a2a ELISA Kit, slc3a2b ELISA Kit

Background:

Gene Name: Solute Carrier Family 3, Member 2

Gene Aliases: SLC3A2, CD98HC, 4F2, 4F2HC, 4T2HC, MDU1, NACAE, Activators Of Dibasic And Neutral Amino Acid Transport, Antigen Identified By Monoclonal Antibodies 4F2, CD98 Heavy Chain

Gene ID: 6520

UniProt: P08195