SNAPIN ELISA Kit

Catalog No. ABIN5658845

Product Details SNAPIN ELISA Kit

Target: SNAPIN (SNAP-Associated Protein (SNAPIN))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of SNAP Associated Protein (SNAPAP). No significant cross-reactivity or interference between SNAP Associated Protein (SNAPAP) and analogues was observed.

Sensitivity: 0.066 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SNAP Associated Protein (SNAPAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SNAP Associated Protein (SNAPAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SNAP Associated Protein (SNAPAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SNAP Associated Protein (SNAPAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SNAP Associated Protein (SNAPAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SNAP Associated Protein (SNAPAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SNAPIN

Target: SNAPIN (SNAP-Associated Protein (SNAPIN))

Alternative Name: SNAP Associated Protein (SNAPIN Products)

Synonyms: SNAPAP ELISA Kit, si:dkey-222b8.2 ELISA Kit, SNAPIN ELISA Kit, BLOC1S7 ELISA Kit, BLOS7 ELISA Kit, 25kDa ELISA Kit, AA407989 ELISA Kit, AV026596 ELISA Kit, Bloc1s7 ELISA Kit, Snap25bp ELISA Kit, Snapap ELISA Kit, SNAP associated protein ELISA Kit, SNAP-associated protein L homeolog ELISA Kit, SNAP-associated protein ELISA Kit, SNAPIN ELISA Kit, snapin.L ELISA Kit, snapin ELISA Kit, Snapin ELISA Kit

Background:

Gene Name: SNAP Associated Protein

Gene Aliases: SNAPIN, SNAP-25-Binding Protein, Biogenesis of lysosome-related organelles complex 1 subunit 7, Synaptosomal-associated protein 25-binding protein

Gene ID: 23557

UniProt: O95295

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis