SUMO1 ELISA Kit

Catalog No. ABIN5658839

Product Details SUMO1 ELISA Kit

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Small Ubiquitin Related Modifier Protein 1 (SUMO1). No significant cross-reactivity or interference between Small Ubiquitin Related Modifier Protein 1 (SUMO1) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Ubiquitin Related Modifier Protein 1 (SUMO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Small Ubiquitin Related Modifier Protein 1 (SUMO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Ubiquitin Related Modifier Protein 1 (SUMO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Small Ubiquitin Related Modifier Protein 1 (SUMO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Small Ubiquitin Related Modifier Protein 1 (SUMO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Small Ubiquitin Related Modifier Protein 1 (SUMO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SUMO1

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Alternative Name: Small Ubiquitin Related Modifier Protein 1 (SUMO1 Products)

Synonyms: DAP1 ELISA Kit, GMP1 ELISA Kit, OFC10 ELISA Kit, PIC1 ELISA Kit, SENP2 ELISA Kit, SMT3 ELISA Kit, SMT3C ELISA Kit, SMT3H3 ELISA Kit, UBL1 ELISA Kit, hm:zeh0670 ELISA Kit, wu:fb74c02 ELISA Kit, wu:fd12c02 ELISA Kit, zgc:65934 ELISA Kit, zgc:85634 ELISA Kit, SUMO1 ELISA Kit, dap1 ELISA Kit, gmp1 ELISA Kit, ofc10 ELISA Kit, pic1 ELISA Kit, senp2 ELISA Kit, smt3 ELISA Kit, smt3c ELISA Kit, sumo-1 ELISA Kit, sumo1 ELISA Kit, ubl1 ELISA Kit, xsumo-1 ELISA Kit, xsumo1 ELISA Kit, ARABIDOPSIS THALIANA SMALL UBIQUITIN-LIKE MODIFIER 1 ELISA Kit, ATSUMO1 ELISA Kit, F10M23.180 ELISA Kit, F10M23_180 ELISA Kit, SMALL UBIQUITIN-LIKE MODIFIER 1 ELISA Kit, SUM1 ELISA Kit, SUMO 1 ELISA Kit, small ubiquitin-like modifier 1 ELISA Kit, pigSUMO1 ELISA Kit, SENTRIN ELISA Kit, SMTP3 ELISA Kit, SUMO-1 ELISA Kit, Smt3C ELISA Kit, Ubl1 ELISA Kit, small ubiquitin-like modifier 1 ELISA Kit, small ubiquitin-like modifier 1 S homeolog ELISA Kit, small ubiquitin-related protein 1 ELISA Kit, SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) ELISA Kit, SMT3 suppressor of mif two 3 homolog 1 (yeast) ELISA Kit, small ubiquitin-like modifier 1 L homeolog ELISA Kit, Sumo1 ELISA Kit, SUMO1 ELISA Kit, sumo1 ELISA Kit, sumo1.S ELISA Kit, sumo-1 ELISA Kit, sumo1.L ELISA Kit

Background:

Gene Name: Small Ubiquitin Related Modifier Protein 1

Gene Aliases: GMP1, OFC10, PIC1, SMT3, SMT3C, SMT3H3, SUMO-1, UBL1, GAP-modifying protein 1, Ubiquitin-homology domain protein PIC1

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway