SMUG1 ELISA Kit

Catalog No. ABIN5658805

Product Details SMUG1 ELISA Kit

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1). No significant cross-reactivity or interference between Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) and analogues was observed.

Sensitivity: 0.051 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SMUG1

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Alternative Name: Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1 Products)

Synonyms: SMUG1 ELISA Kit, xsmug1 ELISA Kit, 1200013B09Rik ELISA Kit, A930006H09Rik ELISA Kit, C85220 ELISA Kit, FDG ELISA Kit, HMUDG ELISA Kit, UNG3 ELISA Kit, single-strand-selective monofunctional uracil-DNA glycosylase 1 ELISA Kit, single-strand selective monofunctional uracil DNA glycosylase ELISA Kit, single-strand-selective monofunctional uracil-DNA glycosylase 1 L homeolog ELISA Kit, SMUG1 ELISA Kit, smug1 ELISA Kit, smuG1 ELISA Kit, smuG ELISA Kit, LOC5577584 ELISA Kit, CpipJ_CPIJ002767 ELISA Kit, Smug1 ELISA Kit, smug1.L ELISA Kit

Background:

Gene Name: Single Strand Selective Monofunctional Uracil DNA Glycosylase 1

Gene Aliases: FDG, HMUDG, UNG3

Gene ID: 23583

UniProt: Q53HV7

Pathways: DNA Damage Repair