SIRPB1 ELISA Kit

Catalog No. ABIN5658791

Product Details SIRPB1 ELISA Kit

Target: SIRPB1 (Signal-Regulatory Protein beta 1 (SIRPB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Signal Regulatory Protein Beta 1 (SIRPb1). No significant cross-reactivity or interference between Signal Regulatory Protein Beta 1 (SIRPb1) and analogues was observed.

Sensitivity: 0.109 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Regulatory Protein Beta 1 (SIRPb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Regulatory Protein Beta 1 (SIRPb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Regulatory Protein Beta 1 (SIRPb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Regulatory Protein Beta 1 (SIRPb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Regulatory Protein Beta 1 (SIRPb1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Regulatory Protein Beta 1 (SIRPb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SIRPB1

Target: SIRPB1 (Signal-Regulatory Protein beta 1 (SIRPB1))

Alternative Name: Signal Regulatory Protein Beta 1 (SIRPB1 Products)

Synonyms: CD172b ELISA Kit, SIRP-BETA-1 ELISA Kit, SIRPB1 ELISA Kit, SIRPG ELISA Kit, signal regulatory protein beta 1 ELISA Kit, signal-regulatory protein beta-1 ELISA Kit, signal-regulatory protein beta-1 isoform 3 ELISA Kit, SIRPB1 ELISA Kit, LOC458036 ELISA Kit, LOC458430 ELISA Kit, LOC485809 ELISA Kit, LOC715912 ELISA Kit, LOC100458151 ELISA Kit

Background:

Gene Name: Signal Regulatory Protein Beta 1

Gene Aliases: CD172-B, CD172b, SIRP-B1, SIRP-beta-1, CD172 antigen-like family member B

Gene ID: 10326

UniProt: O00241