SPINK5 ELISA Kit
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- Target See all SPINK5 products
- SPINK5 (serine Peptidase Inhibitor, Kazal Type 5 (SPINK5))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.312 ng/mL - 20 ng/mL
- Minimum Detection Limit
- 0.312 ng/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Serine Peptidase Inhibitor Kazal Type 5 (SPINK5). No significant cross-reactivity or interference between Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) and analogues was observed.
- Sensitivity
- 0.113 ng/mL
- Featured
- Discover our best selling SPINK5 ELISA Kit
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Peptidase Inhibitor Kazal Type 5 (SPINK5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all SPINK5 products
- SPINK5 (serine Peptidase Inhibitor, Kazal Type 5 (SPINK5))
- Alternative Name
- Serine Peptidase Inhibitor Kazal Type 5 (SPINK5 Products)
- Synonyms
- LEKTI ELISA Kit, LETKI ELISA Kit, NETS ELISA Kit, NS ELISA Kit, VAKTI ELISA Kit, 2310065D10Rik ELISA Kit, AV238912 ELISA Kit, LEKT1 ELISA Kit, LETK1 ELISA Kit, VAKT1 ELISA Kit, serine peptidase inhibitor, Kazal type 5 ELISA Kit, Spink5 ELISA Kit, SPINK5 ELISA Kit
- Background
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Gene Name: Serine Peptidase Inhibitor Kazal Type 5
Gene Aliases: LEKTI, LETKI, NETS, NS, VAKTI, Lymphoepithelial Kazal-Type-Related Inhibitor, Lympho-epithelial Kazal-type-related inhibitor
- Gene ID
- 11005
- UniProt
- Q9NQ38
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