SPTLC3 ELISA Kit

Catalog No. ABIN5658721

Product Details SPTLC3 ELISA Kit

Target: SPTLC3 (Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3). No significant cross-reactivity or interference between Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SPTLC3

Target: SPTLC3 (Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3))

Alternative Name: Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3 Products)

Synonyms: MGC130979 ELISA Kit, SPTLC2L ELISA Kit, RGD1310030 ELISA Kit, C20orf38 ELISA Kit, LCB2B ELISA Kit, LCB3 ELISA Kit, SPT3 ELISA Kit, dJ718P11 ELISA Kit, dJ718P11.1 ELISA Kit, hLCB2b ELISA Kit, C130053K05Rik ELISA Kit, serine palmitoyltransferase long chain base subunit 3 ELISA Kit, serine palmitoyltransferase, long chain base subunit 3 S homeolog ELISA Kit, serine palmitoyltransferase 3 ELISA Kit, serine palmitoyltransferase, long chain base subunit 3 ELISA Kit, SPTLC3 ELISA Kit, sptlc3.S ELISA Kit, LOC100438231 ELISA Kit, sptlc3 ELISA Kit, LOC100546896 ELISA Kit, Sptlc3 ELISA Kit

Background:

Gene Name: Serine Palmitoyltransferase, Long Chain Base Subunit 3

Gene Aliases: SPTLC2L, SPT 3, LCB 3, C20orf38, Aminotransferase 2, Serine-palmitoyl-CoA transferase 3, Long chain base biosynthesis protein 3

Gene ID: 55304

UniProt: Q9NUV7