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SPTLC1 ELISA Kit

SPTLC1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5658719
  • Target See all SPTLC1 ELISA Kits
    SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))
    Reactivity
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1). No significant cross-reactivity or interference between Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) and analogues was observed.
    Sensitivity
    0.054 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all SPTLC1 ELISA Kits
    SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))
    Alternative Name
    Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1 Products)
    Synonyms
    ATSPT1 ELISA Kit, SERINE PALMITOYLTRANSFERASE 1 ELISA Kit, serine palmitoyltransferase 1 ELISA Kit, Afu6g00300 ELISA Kit, HSAN1 ELISA Kit, HSN1 ELISA Kit, LBC1 ELISA Kit, LCB1 ELISA Kit, SPT1 ELISA Kit, SPTI ELISA Kit, wu:fc75h04 ELISA Kit, wu:fp41c08 ELISA Kit, zgc:112247 ELISA Kit, AW552086 ELISA Kit, C77762 ELISA Kit, E030036H05 ELISA Kit, Lcb1 ELISA Kit, RGD1306617 ELISA Kit, Spt1 ELISA Kit, serine palmitoyltransferase 1 ELISA Kit, Serine palmitoyltransferase 1 ELISA Kit, serine palmitoyltransferase long chain base subunit 1 ELISA Kit, serine palmitoyltransferase, long chain base subunit 1 ELISA Kit, serine palmitoyltransferase, long chain base subunit 1 L homeolog ELISA Kit, SPT1 ELISA Kit, AFUA_6G00300 ELISA Kit, PAS_FragB_0040 ELISA Kit, SPTLC1 ELISA Kit, sptlc1 ELISA Kit, sptlc1.L ELISA Kit, Sptlc1 ELISA Kit
    Background

    Gene Name: Serine Palmitoyltransferase, Long Chain Base Subunit 1

    Gene Aliases: HSAN, HSAN1, HSN1, LBC1, LCB1, SPT1, SPTI, Hereditary Sensory Neuropathy,Type 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1

    Gene ID
    268656
    UniProt
    O35704
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