SOSTDC1 ELISA Kit

Catalog No. ABIN5658614

Product Details SOSTDC1 ELISA Kit

Target: SOSTDC1 (Sclerostin Domain Containing 1 (SOSTDC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Sclerostin Domain Containing Protein 1 (SOSTDC1). No significant cross-reactivity or interference between Sclerostin Domain Containing Protein 1 (SOSTDC1) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sclerostin Domain Containing Protein 1 (SOSTDC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sclerostin Domain Containing Protein 1 (SOSTDC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sclerostin Domain Containing Protein 1 (SOSTDC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sclerostin Domain Containing Protein 1 (SOSTDC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sclerostin Domain Containing Protein 1 (SOSTDC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sclerostin Domain Containing Protein 1 (SOSTDC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SOSTDC1

Target: SOSTDC1 (Sclerostin Domain Containing 1 (SOSTDC1))

Alternative Name: Sclerostin Domain Containing Protein 1 (SOSTDC1 Products)

Synonyms: SOSTDC1 ELISA Kit, zgc:110293 ELISA Kit, DKFZp469J0531 ELISA Kit, Usag1 ELISA Kit, ECTODIN ELISA Kit, USAG1 ELISA Kit, 0610006G05Rik ELISA Kit, Sostl ELISA Kit, USAG-1 ELISA Kit, Wise ELISA Kit, ectodin ELISA Kit, WISE ELISA Kit, sclerostin domain containing 1 ELISA Kit, sclerostin domain containing 1a ELISA Kit, SOSTDC1 ELISA Kit, sostdc1a ELISA Kit, Sostdc1 ELISA Kit

Background:

Gene Name: Sclerostin Domain Containing Protein 1

Gene Aliases: CDA019, USAG1, Ectodin, Ectodermal BMP inhibitor, Uterine sensitization-associated gene 1 protein

Gene ID: 25928

UniProt: Q6X4U4