SGCD ELISA Kit

Catalog No. ABIN5658595

Product Details SGCD ELISA Kit

Target: SGCD (Sarcoglycan, delta (35kDa Dystrophin-Associated Glycoprotein) (SGCD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Sarcoglycan Delta (SGCd). No significant cross-reactivity or interference between Sarcoglycan Delta (SGCd) and analogues was observed.

Sensitivity: 0.28 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sarcoglycan Delta (SGCd). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sarcoglycan Delta (SGCd). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sarcoglycan Delta (SGCd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sarcoglycan Delta (SGCd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sarcoglycan Delta (SGCd) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sarcoglycan Delta (SGCd) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SGCD

Target: SGCD (Sarcoglycan, delta (35kDa Dystrophin-Associated Glycoprotein) (SGCD))

Alternative Name: Sarcoglycan Delta (SGCD Products)

Synonyms: BEST:GH21860 ELISA Kit, CG14808 ELISA Kit, CT34621 ELISA Kit, DmSCG-gammadelta ELISA Kit, Dmel\\CG14808 ELISA Kit, EG:4F1.1 ELISA Kit, GH21860 ELISA Kit, Sgcd ELISA Kit, wu:fc49e11 ELISA Kit, zgc:110649 ELISA Kit, 35DAG ELISA Kit, CMD1L ELISA Kit, DAGD ELISA Kit, SG-delta ELISA Kit, SGCDP ELISA Kit, SGD ELISA Kit, Delta-SG ELISA Kit, 35kDa ELISA Kit, delta-SG ELISA Kit, sarcoglycan delta ELISA Kit, Sarcoglycan delta ELISA Kit, sarcoglycan, delta (dystrophin-associated glycoprotein) ELISA Kit, sarcoglycan, delta ELISA Kit, SGCD ELISA Kit, Scgdelta ELISA Kit, sgcd ELISA Kit, Sgcd ELISA Kit

Background:

Gene Name: Sarcoglycan Delta

Gene Aliases: 35DAG, CMD1L, DAGD, SG-delta, SGCDP, SGD, 35 kDa Dystrophin-Associated Glycoprotein

Gene ID: 6444

UniProt: Q92629

Pathways: Maintenance of Protein Location