s100a4 ELISA Kit

Catalog No. ABIN5658559

Product Details s100a4 ELISA Kit

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A4 (S100A4). No significant cross-reactivity or interference between S100 Calcium Binding Protein A4 (S100A4) and analogues was observed.

Sensitivity: 0.32 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A4 (S100A4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A4 (S100A4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A4 (S100A4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A4 (S100A4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A4 (S100A4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A4 (S100A4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100 Calcium Binding Protein A4 (S100A4 Products)

Synonyms: 18A2 ELISA Kit, 42A ELISA Kit, CAPL ELISA Kit, FSP1 ELISA Kit, MTS1 ELISA Kit, P9KA ELISA Kit, PEL98 ELISA Kit, 42a ELISA Kit, Capl ELISA Kit, FSp1 ELISA Kit, Mts1 ELISA Kit, PeL98 ELISA Kit, metastasin ELISA Kit, pk9a ELISA Kit, S100A4 ELISA Kit, P9ka ELISA Kit, RNP9KA ELISA Kit, S100 calcium binding protein A4 ELISA Kit, S100 calcium-binding protein A4 ELISA Kit, S100A4 ELISA Kit, S100a4 ELISA Kit

Background:

Gene Name: S100 Calcium Binding Protein A4

Gene Aliases: S100-A4, MTS1, 18A2, 42A, CAPL, P9KA, PEL98, Calcium protein, Calvasculin, Metastasin, Protein Mts1, Placental calcium-binding protein, Murine placental homolog

Gene ID: 6275

UniProt: P26447

Pathways: S100 Proteins