S100A10 ELISA Kit

Catalog No. ABIN5658546

Product Details S100A10 ELISA Kit

Target: S100A10 (S100 Calcium Binding Protein A10 (S100A10))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A10 (S100A10). No significant cross-reactivity or interference between S100 Calcium Binding Protein A10 (S100A10) and analogues was observed.

Sensitivity: 0.063 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A10 (S100A10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A10 (S100A10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A10 (S100A10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A10 (S100A10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A10 (S100A10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A10 (S100A10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for S100A10

Target: S100A10 (S100 Calcium Binding Protein A10 (S100A10))

Alternative Name: S100 Calcium Binding Protein A10 (S100A10 Products)

Synonyms: 42C ELISA Kit, ANX2L ELISA Kit, ANX2LG ELISA Kit, CAL1L ELISA Kit, CLP11 ELISA Kit, Ca[1] ELISA Kit, GP11 ELISA Kit, P11 ELISA Kit, p10 ELISA Kit, AA409961 ELISA Kit, AL024248 ELISA Kit, CAL12 ELISA Kit, Cal1l ELISA Kit, p11 ELISA Kit, s100a10 ELISA Kit, 42c ELISA Kit, anx2l ELISA Kit, anx2lg ELISA Kit, cal1l ELISA Kit, calpactin ELISA Kit, clp11 ELISA Kit, gp11 ELISA Kit, p11-A ELISA Kit, s100a10b ELISA Kit, hm:zehn1116 ELISA Kit, wu:fa94d11 ELISA Kit, wu:fb05h04 ELISA Kit, zgc:56142 ELISA Kit, zgc:101880 ELISA Kit, S100 calcium binding protein A10 ELISA Kit, S100 calcium binding protein A10 (calpactin) ELISA Kit, S100 calcium binding protein A10 L homeolog ELISA Kit, S100 calcium binding protein A10b ELISA Kit, S100 calcium binding protein A10a ELISA Kit, S100A10 ELISA Kit, S100a10 ELISA Kit, s100a10 ELISA Kit, s100a10.L ELISA Kit, s100a10b ELISA Kit, s100a10a ELISA Kit

Background:

Gene Name: S100 Calcium Binding Protein A10

Gene Aliases: ANX2L, ANX2LG, CAL1L, CLP11, GP11, P11, P10, Calpactin-1 light chain, Cellular ligand of annexin II, Annexin II Ligand,Calpactin I,Light Polypeptide

Gene ID: 6281

UniProt: P60903

Pathways: S100 Proteins