RPS9 ELISA Kit

Catalog No. ABIN5658520

Product Details RPS9 ELISA Kit

Target: RPS9 (Ribosomal Protein S9 (RPS9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ribosomal Protein S9 (RPS9). No significant cross-reactivity or interference between Ribosomal Protein S9 (RPS9) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein S9 (RPS9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribosomal Protein S9 (RPS9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein S9 (RPS9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribosomal Protein S9 (RPS9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribosomal Protein S9 (RPS9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribosomal Protein S9 (RPS9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for RPS9

Target: RPS9 (Ribosomal Protein S9 (RPS9))

Alternative Name: Ribosomal Protein S9 (RPS9 Products)

Synonyms: S9 ELISA Kit, 3010033P07Rik ELISA Kit, AL022771 ELISA Kit, AL022885 ELISA Kit, CG3395 ELISA Kit, Dmel\\CG3395 ELISA Kit, Rp S9 ELISA Kit, RpL11 ELISA Kit, rps9 ELISA Kit, hm:zeh0819 ELISA Kit, zgc:73129 ELISA Kit, zgc:77664 ELISA Kit, ribosomal protein S9 ELISA Kit, 30S ribosomal protein S9 ELISA Kit, Ribosomal protein S9 ELISA Kit, 40S ribosomal protein S9 ELISA Kit, ribosomal protein S9 L homeolog ELISA Kit, RPS9 ELISA Kit, Rps9 ELISA Kit, rps9 ELISA Kit, RpS9 ELISA Kit, rps-9 ELISA Kit, rps9.L ELISA Kit

Background:

Gene Name: Ribosomal Protein S9

Gene Aliases: 40S Ribosomal Protein S9

Gene ID: 6203

UniProt: P46781