RPS6KB1 ELISA Kit

Catalog No. ABIN5658519

Product Details RPS6KB1 ELISA Kit

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1). No significant cross-reactivity or interference between Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Alternative Name: Ribosomal Protein S6 Kinase Beta 1 (RPS6KB1 Products)

Synonyms: PS6K ELISA Kit, S6K ELISA Kit, S6K-beta-1 ELISA Kit, S6K1 ELISA Kit, STK14A ELISA Kit, p70 S6KA ELISA Kit, p70(S6K)-alpha ELISA Kit, p70-S6K ELISA Kit, p70-alpha ELISA Kit, 10539 ELISA Kit, 7084 ELISA Kit, CG10539 ELISA Kit, DS6K ELISA Kit, Dmel\\CG10539 ELISA Kit, Dp70S6k ELISA Kit, Dp70[s6k] ELISA Kit, Dp70s6k ELISA Kit, S6k ELISA Kit, dS6K ELISA Kit, dS6k ELISA Kit, dp70[S6k] ELISA Kit, dp70s6k ELISA Kit, dps6k ELISA Kit, ds6k ELISA Kit, fs(3)07084 ELISA Kit, l(3)07084 ELISA Kit, p70 S6K ELISA Kit, p70/S6K ELISA Kit, p70S6K ELISA Kit, p70[S6 kinase] ELISA Kit, p70[S6K] ELISA Kit, p70[S6k] ELISA Kit, p70[S6kinase] ELISA Kit, p70s6K ELISA Kit, s6k ELISA Kit, s6k11 ELISA Kit, 2610318I15Rik ELISA Kit, 4732464A07Rik ELISA Kit, 70kDa ELISA Kit, AA959758 ELISA Kit, AI256796 ELISA Kit, AI314060 ELISA Kit, p70/85s6k ELISA Kit, p70s6k ELISA Kit, p70s6k-A ELISA Kit, p70-s6k ELISA Kit, ps6k ELISA Kit, rps6kb1 ELISA Kit, rps6kb1-A ELISA Kit, s6K1 ELISA Kit, stk14a ELISA Kit, fc51h01 ELISA Kit, wu:fc51h01 ELISA Kit, zgc:55713 ELISA Kit, ribosomal protein S6 kinase B1 ELISA Kit, Ribosomal protein S6 kinase ELISA Kit, ribosomal protein S6 kinase, polypeptide 1 ELISA Kit, ribosomal protein S6 kinase B1 L homeolog ELISA Kit, ribosomal protein S6 kinase B1 S homeolog ELISA Kit, ribosomal protein S6 kinase b, polypeptide 1b ELISA Kit, RPS6KB1 ELISA Kit, S6k ELISA Kit, Rps6kb1 ELISA Kit, rps6kb1.L ELISA Kit, rps6kb1.S ELISA Kit, rps6kb1b ELISA Kit

Background:

Gene Name: Ribosomal Protein S6 Kinase Beta 1

Gene Aliases: PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha, P70-S6 Kinase 1, 70 kDa ribosomal protein S6 kinase 1, Serine/threonine-protein kinase 14A

Gene ID: 6198

UniProt: P23443

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect