RPL13A ELISA Kit

Catalog No. ABIN5658514

Product Details RPL13A ELISA Kit

Target: RPL13A (Ribosomal Protein L13a (RPL13A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ribosomal Protein L13A (RPL13A). No significant cross-reactivity or interference between Ribosomal Protein L13A (RPL13A) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein L13A (RPL13A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribosomal Protein L13A (RPL13A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein L13A (RPL13A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribosomal Protein L13A (RPL13A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribosomal Protein L13A (RPL13A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribosomal Protein L13A (RPL13A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for RPL13A

Target: RPL13A (Ribosomal Protein L13a (RPL13A))

Alternative Name: Ribosomal Protein L13A (RPL13A Products)

Synonyms: 1810026N22Rik ELISA Kit, Tstap198-7 ELISA Kit, tum-antigen ELISA Kit, L13A ELISA Kit, TSTA1 ELISA Kit, CG1475 ELISA Kit, Dmel\\CG1475 ELISA Kit, L13a2 ELISA Kit, M(3)83B ELISA Kit, Rp L13A ELISA Kit, anon-EST:Posey125 ELISA Kit, anon-EST:fe1A5 ELISA Kit, bs25f04.y1 ELISA Kit, cg1475 ELISA Kit, MGC54018 ELISA Kit, GB16111 ELISA Kit, hm:zehp0384 ELISA Kit, wu:fa95e06 ELISA Kit, wu:fb08g07 ELISA Kit, wu:fd05d08 ELISA Kit, DDBDRAFT_0217704 ELISA Kit, DDBDRAFT_0231192 ELISA Kit, DDB_0217704 ELISA Kit, DDB_0231192 ELISA Kit, ribosomal protein L13A ELISA Kit, ribosomal protein L13a ELISA Kit, Ribosomal protein L13A ELISA Kit, ribosomal protein L13a S homeolog ELISA Kit, 60S ribosomal protein L13a ELISA Kit, ribosomal protein 13a ELISA Kit, ribosomal 60S subunit protein L13A ELISA Kit, Ribosomal protein L13a, component of cytosolic 80S ribosome and 60S large subunit ELISA Kit, S60 ribosomal protein L13a ELISA Kit, Rpl13a ELISA Kit, RPL13A ELISA Kit, RpL13A ELISA Kit, rpl13a.S ELISA Kit, rpl13a ELISA Kit, LOC551418 ELISA Kit, LOC663151 ELISA Kit, RPL13a ELISA Kit

Background:

Gene Name: Ribosomal Protein L13A

Gene Aliases: TSTA1, Tissue Specific Transplantation Antigen 1, 60S ribosomal protein L13a, 23 kDa highly basic protein