ARHGEF7 ELISA Kit

Catalog No. ABIN5658488

Product Details ARHGEF7 ELISA Kit

Target: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7). No significant cross-reactivity or interference between Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) and analogues was observed.

Sensitivity: 0.064 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ARHGEF7

Target: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Alternative Name: Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7 Products)

Synonyms: BETA-PIX ELISA Kit, COOL-1 ELISA Kit, COOL1 ELISA Kit, Nbla10314 ELISA Kit, P50 ELISA Kit, P50BP ELISA Kit, P85 ELISA Kit, P85COOL1 ELISA Kit, P85SPR ELISA Kit, PAK3 ELISA Kit, PIXB ELISA Kit, cool1 ELISA Kit, p50 ELISA Kit, p85 ELISA Kit, pak3 ELISA Kit, pixb ELISA Kit, ARHGEF7 ELISA Kit, P85spr ELISA Kit, Pak3bp ELISA Kit, Cool ELISA Kit, PIX ELISA Kit, betaPix ELISA Kit, betaPix-b ELISA Kit, betaPix-c ELISA Kit, cool-1 ELISA Kit, mKIAA0142 ELISA Kit, p85Cool1 ELISA Kit, p85SPR ELISA Kit, arhgef7 ELISA Kit, bbh ELISA Kit, bpix ELISA Kit, im:7142367 ELISA Kit, wu:fc35h08 ELISA Kit, zgc:101775 ELISA Kit, Rho guanine nucleotide exchange factor 7 ELISA Kit, Rho guanine nucleotide exchange factor 7 S homeolog ELISA Kit, ankyrin repeat domain 10 ELISA Kit, Rho guanine nucleotide exchange factor (GEF7) ELISA Kit, Rho guanine nucleotide exchange factor (GEF) 7b ELISA Kit, ARHGEF7 ELISA Kit, arhgef7.S ELISA Kit, arhgef7 ELISA Kit, ANKRD10 ELISA Kit, Arhgef7 ELISA Kit, arhgef7b ELISA Kit

Background:

Gene Name: Rho Guanine Nucleotide Exchange Factor 7

Gene Aliases: PAK3, BETA-PIX, COOL1, P50, P50BP, P85, P85SPR, PIXB, SH3 Domain-Containing Proline-Rich Protein, PAK-Interacting Exchange Factor Beta

Gene ID: 114559

UniProt: O55043

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation