ARHGDIB ELISA Kit

Catalog No. ABIN5658487

Product Details ARHGDIB ELISA Kit

Target: ARHGDIB (rho GDP Dissociation Inhibitor (GDI) beta (ARHGDIB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Rho GDP Dissociation Inhibitor Beta (ARHGDIb). No significant cross-reactivity or interference between Rho GDP Dissociation Inhibitor Beta (ARHGDIb) and analogues was observed.

Sensitivity: 0.241 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho GDP Dissociation Inhibitor Beta (ARHGDIb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rho GDP Dissociation Inhibitor Beta (ARHGDIb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rho GDP Dissociation Inhibitor Beta (ARHGDIb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rho GDP Dissociation Inhibitor Beta (ARHGDIb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho GDP Dissociation Inhibitor Beta (ARHGDIb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho GDP Dissociation Inhibitor Beta (ARHGDIb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ARHGDIB

Target: ARHGDIB (rho GDP Dissociation Inhibitor (GDI) beta (ARHGDIB))

Alternative Name: Rho GDP Dissociation Inhibitor Beta (ARHGDIB Products)

Synonyms: ARHGDIB ELISA Kit, zgc:65966 ELISA Kit, arhgdib ELISA Kit, MGC69067 ELISA Kit, gdia2 ELISA Kit, rhodgi-2 ELISA Kit, rhodgi2 ELISA Kit, D4 ELISA Kit, GDIA2 ELISA Kit, GDID4 ELISA Kit, LYGDI ELISA Kit, Ly-GDI ELISA Kit, RAP1GN1 ELISA Kit, RhoGDI2 ELISA Kit, Gdid4 ELISA Kit, Rho GDP dissociation inhibitor (GDI) gamma ELISA Kit, Rho GDP dissociation inhibitor (GDI) beta S homeolog ELISA Kit, Rho GDP dissociation inhibitor beta ELISA Kit, Rho GDP dissociation inhibitor (GDI) beta ELISA Kit, Rho, GDP dissociation inhibitor (GDI) beta ELISA Kit, arhgdig ELISA Kit, arhgdib.S ELISA Kit, ARHGDIB ELISA Kit, arhgdib ELISA Kit, Arhgdib ELISA Kit

Background:

Gene Name: Rho GDP Dissociation Inhibitor Beta

Gene Aliases: D4, GDIA2, GDID4, LYGDI, Ly-GDI, RAP1GN1, RhoGDI2

Gene ID: 397

UniProt: P52566

Pathways: Caspase Cascade in Apoptosis