RBP1 CLIA Kit

Catalog No. ABIN5658473

Product Details RBP1 CLIA Kit

Target: RBP1 (Retinol Binding Protein 1, Cellular (RBP1))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 200 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Retinol Binding Protein 1, Cellular (RBP1). No significant cross-reactivity or interference between Retinol Binding Protein 1, Cellular (RBP1) and analogues was observed.

Sensitivity: 0.12 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Retinol Binding Protein 1, Cellular (RBP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Retinol Binding Protein 1, Cellular (RBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Retinol Binding Protein 1, Cellular (RBP1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinol Binding Protein 1, Cellular (RBP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinol Binding Protein 1, Cellular (RBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: RBP1 (Retinol Binding Protein 1, Cellular (RBP1))

Alternative Name: Retinol Binding Protein 1, Cellular (RBP1 Products)

Synonyms: RBBP-1 CLIA Kit, RBBP1 CLIA Kit, RBP-1 CLIA Kit, RBP1 CLIA Kit, CRABP-I CLIA Kit, CRBP CLIA Kit, CRBP1 CLIA Kit, CRBPI CLIA Kit, RBPC CLIA Kit, cb465 CLIA Kit, rbp1 CLIA Kit, wu:fb75e07 CLIA Kit, zgc:73335 CLIA Kit, CRABP1 CLIA Kit, Crbp CLIA Kit, Rbp-1 CLIA Kit, rbp1b CLIA Kit, sb:eu611 CLIA Kit, zgc:100825 CLIA Kit, AT-rich interaction domain 4A CLIA Kit, retinol binding protein 1 CLIA Kit, retinol binding protein 1a, cellular CLIA Kit, retinol binding protein 1 L homeolog CLIA Kit, retinol binding protein 1, cellular CLIA Kit, retinol binding protein 1b, cellular CLIA Kit, ARID4A CLIA Kit, RBP1 CLIA Kit, rbp5 CLIA Kit, rbp1.L CLIA Kit, rbp1 CLIA Kit, Rbp1 CLIA Kit

Background:

Gene Name: Retinol Binding Protein 1, Cellular

Gene Aliases: RBP-1, CRABP-I, CRBP, CRBP1, RBPC, Cellular retinol-binding protein I

Gene ID: 5947

UniProt: P09455