Retinoid X Receptor gamma ELISA Kit

Catalog No. ABIN5658472

Product Details Retinoid X Receptor gamma ELISA Kit

Target: Retinoid X Receptor gamma (RXRG) (Retinoid X Receptor, gamma (RXRG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Retinoid X Receptor Gamma (RXRg). No significant cross-reactivity or interference between Retinoid X Receptor Gamma (RXRg) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoid X Receptor Gamma (RXRg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoid X Receptor Gamma (RXRg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoid X Receptor Gamma (RXRg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinoid X Receptor Gamma (RXRg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinoid X Receptor Gamma (RXRg) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinoid X Receptor Gamma (RXRg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Retinoid X Receptor gamma

Target: Retinoid X Receptor gamma (RXRG) (Retinoid X Receptor, gamma (RXRG))

Alternative Name: Retinoid X Receptor Gamma (RXRG Products)

Synonyms: NR2B3 ELISA Kit, RXRC ELISA Kit, zgc:92183 ELISA Kit, RXRgamma ELISA Kit, RXRG ELISA Kit, RXR-gamma ELISA Kit, nr2b3 ELISA Kit, xrxrg ELISA Kit, rxrc ELISA Kit, Nr2b3 ELISA Kit, NR2B1 ELISA Kit, RXR ELISA Kit, rxra ELISA Kit, rxrg ELISA Kit, retinoid X receptor gamma ELISA Kit, retinoid X receptor, gamma b ELISA Kit, retinoid X receptor gamma L homeolog ELISA Kit, retinoid x receptor, gamma a ELISA Kit, RXRG ELISA Kit, rxrgb ELISA Kit, rxrg.L ELISA Kit, rxrg ELISA Kit, Rxrg ELISA Kit, RXRGAMMA ELISA Kit, rxrga ELISA Kit

Background:

Gene Name: Retinoid X Receptor Gamma

Gene Aliases: NR2B3, RXRC, Nuclear receptor subfamily 2 group B member 3, Retinoic acid receptor RXR-gamma

Gene ID: 6258

UniProt: P48443

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway