Retinoid X Receptor alpha ELISA Kit

Catalog No. ABIN5658470

Product Details Retinoid X Receptor alpha ELISA Kit

Target: Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Retinoid X Receptor Alpha (RXRa). No significant cross-reactivity or interference between Retinoid X Receptor Alpha (RXRa) and analogues was observed.

Sensitivity: 0.117 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoid X Receptor Alpha (RXRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoid X Receptor Alpha (RXRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoid X Receptor Alpha (RXRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinoid X Receptor Alpha (RXRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinoid X Receptor Alpha (RXRa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinoid X Receptor Alpha (RXRa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Retinoid X Receptor alpha

Target: Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))

Alternative Name: Retinoid X Receptor Alpha (RXRA Products)

Synonyms: NR2B1 ELISA Kit, 9530071D11Rik ELISA Kit, Nr2b1 ELISA Kit, RXRalpha1 ELISA Kit, RXRalpha ELISA Kit, rxra-A ELISA Kit, xRXR alpha ELISA Kit, xrxra ELISA Kit, RXRA ELISA Kit, RXR alpha ELISA Kit, etID309731.5 ELISA Kit, rxr ELISA Kit, rxra ELISA Kit, rxrg ELISA Kit, RXRalpha-B ELISA Kit, retinoid X receptor alpha ELISA Kit, retinoid X receptor alpha L homeolog ELISA Kit, retinoid x receptor, alpha b ELISA Kit, retinoid X receptor, alpha a ELISA Kit, RXRA ELISA Kit, Rxra ELISA Kit, rxra.L ELISA Kit, CpipJ_CPIJ010249 ELISA Kit, rxrab ELISA Kit, rxraa ELISA Kit

Background:

Gene Name: Retinoid X Receptor Alpha

Gene Aliases: NR2B1, RXR-A, NR2-B1, Nuclear Receptor Subfamily 2,Group B,Member 1, Retinoic acid receptor RXR-alpha

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Hepatitis C