Retinoic Acid Receptor alpha ELISA Kit

Catalog No. ABIN5658465

Product Details Retinoic Acid Receptor alpha ELISA Kit

Target: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Retinoic Acid Receptor Alpha (RARa). No significant cross-reactivity or interference between Retinoic Acid Receptor Alpha (RARa) and analogues was observed.

Sensitivity: 0.114 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoic Acid Receptor Alpha (RARa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoic Acid Receptor Alpha (RARa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoic Acid Receptor Alpha (RARa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinoic Acid Receptor Alpha (RARa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinoic Acid Receptor Alpha (RARa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinoic Acid Receptor Alpha (RARa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Retinoic Acid Receptor alpha

Target: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Alternative Name: Retinoic Acid Receptor Alpha (RARA Products)

Synonyms: NR1B1 ELISA Kit, RAR ELISA Kit, Nr1b1 ELISA Kit, RARalpha1 ELISA Kit, rar-alpha ELISA Kit, etID309833.12 ELISA Kit, rara ELISA Kit, rara2a ELISA Kit, zRAR ELISA Kit, zRAR-alpha ELISA Kit, zgc:109797 ELISA Kit, nr1b1 ELISA Kit, RAR-ALPHA1 ELISA Kit, HS-RARa ELISA Kit, fj66e06 ELISA Kit, rara2b ELISA Kit, wu:fj66e06 ELISA Kit, retinoic acid receptor alpha ELISA Kit, retinoic acid receptor, alpha ELISA Kit, retinoic acid receptor, alpha a ELISA Kit, retinoic acid receptor alpha L homeolog ELISA Kit, retinoic acid receptor, alpha b ELISA Kit, RARA ELISA Kit, Rara ELISA Kit, LOC100136372 ELISA Kit, raraa ELISA Kit, rara.L ELISA Kit, rara ELISA Kit, rarab ELISA Kit

Background:

Gene Name: Retinoic Acid Receptor Alpha

Gene Aliases: RAR-A, NR1B1, RAR, RAR-Alpha, Nuclear Receptor Subfamily 1 Group B Member 1

Gene ID: 24705

UniProt: Q499N1

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, S100 Proteins