RNLS ELISA Kit

Catalog No. ABIN5658434

Product Details RNLS ELISA Kit

Target: RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Renalase (RNLS). No significant cross-reactivity or interference between Renalase (RNLS) and analogues was observed.

Sensitivity: 0.67 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Renalase (RNLS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Renalase (RNLS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Renalase (RNLS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Renalase (RNLS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renalase (RNLS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renalase (RNLS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for RNLS

Target: RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))

Alternative Name: Renalase (RNLS Products)

Synonyms: zgc:92286 ELISA Kit, C10orf59 ELISA Kit, RENALASE ELISA Kit, 6530404N21Rik ELISA Kit, AI452315 ELISA Kit, AW060440 ELISA Kit, RGD1309804 ELISA Kit, renalase, FAD dependent amine oxidase ELISA Kit, renalase, FAD-dependent amine oxidase ELISA Kit, RNLS ELISA Kit, rnls ELISA Kit, Rnls ELISA Kit

Background:

Gene Name: Renalase

Gene Aliases: MAO-C, C10orf59, FAD-Dependent Amine Oxidase, Monoamine oxidase-C, alpha-NAD(P)H oxidase/anomerase

Gene ID: 361751

UniProt: Q5U2W9