RGS1 ELISA Kit

Catalog No. ABIN5658415

Product Details RGS1 ELISA Kit

Target: RGS1 (Regulator of G-Protein Signaling 1 (RGS1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Regulator Of G Protein Signaling 1 (RGS1). No significant cross-reactivity or interference between Regulator Of G Protein Signaling 1 (RGS1) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulator Of G Protein Signaling 1 (RGS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regulator Of G Protein Signaling 1 (RGS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulator Of G Protein Signaling 1 (RGS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regulator Of G Protein Signaling 1 (RGS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulator Of G Protein Signaling 1 (RGS1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulator Of G Protein Signaling 1 (RGS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for RGS1

Target: RGS1 (Regulator of G-Protein Signaling 1 (RGS1))

Alternative Name: Regulator Of G Protein Signaling 1 (RGS1 Products)

Synonyms: 1R20 ELISA Kit, BL34 ELISA Kit, IER1 ELISA Kit, IR20 ELISA Kit, RGS1 ELISA Kit, 1r20 ELISA Kit, bl34 ELISA Kit, ier1 ELISA Kit, ir20 ELISA Kit, regulator of G protein signaling 1 ELISA Kit, regulator of G-protein signaling 1 ELISA Kit, regulator of G-protein signaling 1 S homeolog ELISA Kit, Regulator of G-protein signaling 1 ELISA Kit, RGS1 ELISA Kit, Rgs1 ELISA Kit, rgs1.S ELISA Kit, rgs1 ELISA Kit

Background:

Gene Name: Regulator Of G Protein Signaling 1

Gene Aliases: 1R20, BL34, IER1, IR20, B-cell activation protein BL34, Early response protein 1R20

Gene ID: 5996

UniProt: Q08116

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events