Fc gamma RII (CD32) ELISA Kit

Catalog No. ABIN5658377

Product Details

Target: Fc gamma RII (CD32)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.88 pg/mL - 3000 pg/mL

Minimum Detection Limit: 46.88 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Receptor II For The Fc Region Of Immunoglobulin G (FcgRII). No significant cross-reactivity or interference between Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) and analogues was observed.

Sensitivity: 17.35 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor II For The Fc Region Of Immunoglobulin G (FcgRII). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor II For The Fc Region Of Immunoglobulin G (FcgRII). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor II For The Fc Region Of Immunoglobulin G (FcgRII), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: Receptor II For The Fc Region Of Immunoglobulin G (CD32 Products)

Synonyms: CD32 ELISA Kit, CD32B ELISA Kit, FCG2 ELISA Kit, FCGR2 ELISA Kit, IGFR2 ELISA Kit, FCGR2B ELISA Kit, AI528646 ELISA Kit, F630109E10Rik ELISA Kit, Fc[g]RII ELISA Kit, FcgRII ELISA Kit, Fcgr2 ELISA Kit, Fcgr2a ELISA Kit, Fcr-2 ELISA Kit, Fcr-3 ELISA Kit, Ly-17 ELISA Kit, Ly-m20 ELISA Kit, LyM-1 ELISA Kit, Lym-1 ELISA Kit, FCRII ELISA Kit, FcRII ELISA Kit, Fc fragment of IgG receptor IIb ELISA Kit, Fc receptor, IgG, low affinity IIb ELISA Kit, FCGR2B ELISA Kit, Fcgr2b ELISA Kit

Target Type: Antibody

Background:

Gene Name: Receptor II For The Fc Region Of Immunoglobulin G

Gene Aliases: CD32, FCGR2A, FCG2, FCGR2A1, FCGR2, FCGR2B, FCGR2C, Fc Fragment Of IgG, Low Affinity IIa, Receptor, Low affinity immunoglobulin gamma Fc region receptor II-a

Gene ID: 2212

UniProt: P12318