FCGR1 ELISA Kit

Catalog No. ABIN5658374

Product Details FCGR1 ELISA Kit

Target: FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Receptor I For The Fc Region Of Immunoglobulin G (FcgRI). No significant cross-reactivity or interference between Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) and analogues was observed.

Sensitivity: 0.257 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor I For The Fc Region Of Immunoglobulin G (FcgRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FCGR1

Target: FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))

Alternative Name: Receptor I For The Fc Region Of Immunoglobulin G (FCGR1 Products)

Synonyms: CD64 ELISA Kit, CD64A ELISA Kit, FCRI ELISA Kit, IGFR1 ELISA Kit, AI323638 ELISA Kit, AV092959 ELISA Kit, FcgammaRI ELISA Kit, IGGHAFC ELISA Kit, FCGR1A ELISA Kit, Fcgr1 ELISA Kit, Fcgr1b ELISA Kit, Fc fragment of IgG receptor Ia ELISA Kit, Fc receptor, IgG, high affinity I ELISA Kit, FCGR1A ELISA Kit, Fcgr1 ELISA Kit, Fcgr1a ELISA Kit

Background:

Gene Name: Receptor I For The Fc Region Of Immunoglobulin G

Gene Aliases: CD64, FCGR1, FCGR1A, FCGR1B, FCGR1C, FcgRIA, CD64A, FcgRIB, CD64B, FcgRIC, CD64C, High affinity immunoglobulin gamma Fc receptor I

Gene ID: 14129

UniProt: P26151

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process