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FCGR1 ELISA Kit

FCGR1 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5658373
  • Target See all FCGR1 ELISA Kits
    FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))
    Reactivity
    • 2
    • 2
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78 pg/mL - 5000 pg/mL
    Minimum Detection Limit
    78 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Receptor I For The Fc Region Of Immunoglobulin G (FcgRI). No significant cross-reactivity or interference between Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) and analogues was observed.
    Sensitivity
    31 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor I For The Fc Region Of Immunoglobulin G (FcgRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all FCGR1 ELISA Kits
    FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))
    Alternative Name
    Receptor I For The Fc Region Of Immunoglobulin G (FCGR1 Products)
    Synonyms
    CD64 ELISA Kit, CD64A ELISA Kit, FCRI ELISA Kit, IGFR1 ELISA Kit, AI323638 ELISA Kit, AV092959 ELISA Kit, FcgammaRI ELISA Kit, IGGHAFC ELISA Kit, FCGR1A ELISA Kit, Fcgr1 ELISA Kit, Fcgr1b ELISA Kit, Fc fragment of IgG receptor Ia ELISA Kit, Fc receptor, IgG, high affinity I ELISA Kit, FCGR1A ELISA Kit, Fcgr1 ELISA Kit, Fcgr1a ELISA Kit
    Background

    Gene Name: Receptor I For The Fc Region Of Immunoglobulin G

    Gene Aliases: CD64, FCGR1, FCGR1A, FCGR1B, FCGR1C, FcgRIA, CD64A, FcgRIB, CD64B, FcgRIC, CD64C, High affinity immunoglobulin gamma Fc receptor I

    Gene ID
    2209
    UniProt
    P12314
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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