ROMO1 ELISA Kit

Catalog No. ABIN5658361

Product Details ROMO1 ELISA Kit

Target: ROMO1 (Reactive Oxygen Species Modulator 1 (ROMO1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Reactive Oxygen Species Modulator 1 (ROMO1). No significant cross-reactivity or interference between Reactive Oxygen Species Modulator 1 (ROMO1) and analogues was observed.

Sensitivity: 0.053 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Reactive Oxygen Species Modulator 1 (ROMO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Reactive Oxygen Species Modulator 1 (ROMO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Reactive Oxygen Species Modulator 1 (ROMO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Reactive Oxygen Species Modulator 1 (ROMO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Reactive Oxygen Species Modulator 1 (ROMO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Reactive Oxygen Species Modulator 1 (ROMO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ROMO1

Target: ROMO1 (Reactive Oxygen Species Modulator 1 (ROMO1))

Alternative Name: Reactive Oxygen Species Modulator 1 (ROMO1 Products)

Synonyms: romo1 ELISA Kit, zgc:77111 ELISA Kit, C20orf52 ELISA Kit, mtgmp ELISA Kit, RA_r333_jsm669B4f ELISA Kit, MTGM ELISA Kit, MTGMP ELISA Kit, bA353C18.2 ELISA Kit, 2010100O12Rik ELISA Kit, AI853864 ELISA Kit, C13H20ORF52 ELISA Kit, MGR2 ELISA Kit, reactive oxygen species modulator 1 ELISA Kit, reactive oxygen species modulator 1 L homeolog ELISA Kit, romo1 ELISA Kit, ROMO1 ELISA Kit, Romo1 ELISA Kit, romo1.L ELISA Kit

Background:

Gene Name: Reactive Oxygen Species Modulator 1

Gene Aliases: C20orf52, MTGMP, Glyrichin, Mitochondrial Targeting GXXXG Protein, Epididymis tissue protein Li 175, Protein MGR2 homolog